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Jason Sheltzer Lab: CRISPR plasmids to knock out SARS-CoV-2 host genes

Unpublished

Plasmids from Article

ID Plasmid Purpose
153011ACE2 g1 + Cas9A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2
153012ACE2 g2A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry
153013ACE2 g3A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry
153014ACE2 g4 + Cas9A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2
153015TMPRSS2 g1 + Cas9A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2
153016TMPRSS2 g2A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing mCherry
153017TMPRSS2 g3A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing mCherry
153018TMPRSS2 g4 + Cas9A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2

Antibodies from Article