Paired guide RNA CRISPR-Cas9 screening for protein-coding genes and lncRNAs involved in transdifferentiation of human B-cells to macrophages.
Arnan C, Ullrich S, Pulido-Quetglas C, Nurtdinov R, Esteban A, Blanco-Fernandez J, Aparicio-Prat E, Johnson R, Perez-Lluch S, Guigo R
BMC Genomics. 2022 May 26;23(1):402. doi: 10.1186/s12864-022-08612-7.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 140098 | pDECKO_mCherry_Rat_cebp-alfa | CRISPR-Cas9 library validation (positive control) |
| 140099 | pDECKO_mCherry_SPI1 | CRISPR-Cas9 library validation (positive control) |
| 140100 | pDECKO_mCherry_Intergenic | CRISPR-Cas9 library validation (negative control) |
| 140101 | pDECKO_mCherry_CD19.4 | CRISPR-Cas9 Knock down |
| 140102 | pDECKO_mCherry_CEACAM1 | CRISPR-Cas9 library validation |
| 140103 | pDECKO_mCherry_FURIN | CRISPR-Cas9 library validation |
| 140104 | pDECKO_mCherry_KLF4 | CRISPR-Cas9 library validation |
| 140105 | pDECKO_mCherry_NFE2 | CRISPR-Cas9 Knock down |
| 140106 | pDECKO_mCherry_TREML2 | CRISPR-Cas9 Knock down |
| 140107 | pDECKO_mCherry_LINC02432 | CRISPR-Cas9 library validation |
| 140108 | pDECKO_mCherry_MIR3945HG | CRISPR-Cas9 library validation |
| 140109 | pDECKO_mCherry_non-targeting | CRISPR-Cas9 library validation (negative control) |
| 183825 | BLaER1 pgRNA CRISPR library | CRISPR-Cas9 library to target lncRNAs and protein coding genes with a potential role in B-cell to macrophage transdifferentiation. |