A genome-scale CRISPR interference guide library enables comprehensive phenotypic profiling in yeast.
McGlincy NJ, Meacham ZA, Reynaud KK, Muller R, Baum R, Ingolia NT
BMC Genomics. 2021 Mar 23;22(1):205. doi: 10.1186/s12864-021-07518-0.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
139474 | pNTI647 dCas9-Mxi1 TetR KanMX | Integrates CRISPRi effector and tet-responsive regulator |
139475 | pNTI661 pRPR1(TetO)-sgRNA | Vector for tet-regulated sgRNA expression in yeast |
161769 | Ingolia lab S cerevisiae CRISPRi v1 – barcodes 181005 | Inducible CRISPRi library |