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ER-lysosome contacts enable cholesterol sensing by mTORC1 and drive aberrant growth signalling in Niemann-Pick type C.

Lim CY, Davis OB, Shin HR, Zhang J, Berdan CA, Jiang X, Counihan JL, Ory DS, Nomura DK, Zoncu R
Nat Cell Biol. 2019 Oct;21(10):1206-1218. doi: 10.1038/s41556-019-0391-5. Epub 2019 Sep 23. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
134604pGEX-KG-D4H*-mCherryfluorescent cholesterol-binding probe
134630pLJM1-FLAG-GFP-Tmem192lysosomal marker
134631pLJM1-Tmem192-mRFP-3xHAbait for affinity-isolation of lysosomes
134645pLJM1-Lyso-FLAG-GFP-Sac1-Cat-WTlysosome-targeted Sac1 catalytic domain wild type
134653pLJM1-Lyso-FLAG-GFP-Sac1-Cat-CSlysosome-targeted Sac1 catalytic domain C389S
134659pLJM1-FLAG-GFP-OSBPFlag and GFP-tagged OSBP

Antibodies from Article