ER-lysosome contacts enable cholesterol sensing by mTORC1 and drive aberrant growth signalling in Niemann-Pick type C.
Lim CY, Davis OB, Shin HR, Zhang J, Berdan CA, Jiang X, Counihan JL, Ory DS, Nomura DK, Zoncu R
Nat Cell Biol. 2019 Oct;21(10):1206-1218. doi: 10.1038/s41556-019-0391-5. Epub 2019 Sep 23.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
134604 | pGEX-KG-D4H*-mCherry | fluorescent cholesterol-binding probe |
134630 | pLJM1-FLAG-GFP-Tmem192 | lysosomal marker |
134631 | pLJM1-Tmem192-mRFP-3xHA | bait for affinity-isolation of lysosomes |
134645 | pLJM1-Lyso-FLAG-GFP-Sac1-Cat-WT | lysosome-targeted Sac1 catalytic domain wild type |
134653 | pLJM1-Lyso-FLAG-GFP-Sac1-Cat-CS | lysosome-targeted Sac1 catalytic domain C389S |
134659 | pLJM1-FLAG-GFP-OSBP | Flag and GFP-tagged OSBP |