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A general approach to engineer positive-going eFRET voltage indicators.

Abdelfattah AS, Valenti R, Zheng J, Wong A, Podgorski K, Koyama M, Kim DS, Schreiter ER
Nat Commun. 2020 Jul 10;11(1):3444. doi: 10.1038/s41467-020-17322-1. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
129253pCAG-PositronExpress Positron in mammalian cells
129254pCAG-Positron-STExpress soma-localized Positron in mammalian cells
129255pCAG-Ace1_Q89D_D100N_E206V-HaloTagExpress Ace1_Q89D_D100N_E206V-HaloTag in mammalian cells
129256pCAG-Ace2_D92N_E199V-mNeonExpress Ace2_D92N_E199V-mNeon in mammalian cells
129257pCAG-Ace2_D92N_E199V-mRuby3Express Ace2_D92N_E199V-mRuby3 in mammalian cells
129258pCAG-QuaAr3_Q95D_H106N_E214V-HaloTagExpress QuaAr3_Q95D_H106N_E214V-HaloTag in mammalian cells
129259pCAG-Mac_Q139D_D150N_E258V-HaloTagExpress Mac_Q139D_D150N_E258V-HaloTag in mammalian cells
129260pCAG-Ace1_Q89D_D100N_E206V-HaloTag-STExpress soma-localized Ace1_Q89D_D100N_E206V-HaloTag in mammalian cells
129261pCAG-Ace2_D92N_E199V-mNeon-STExpress soma-localized Ace2_D92N_E199V-mNeon in mammalian cells
129262pCAG-Ace2_D92N_E199V-mRuby3-STExpress soma-localized Ace2_D92N_E199V-mRuby3 in mammalian cells
129263pCAG-QuaAr3_Q95D_H106N_E214V-HaloTag-STExpress soma-localized QuaAr3_Q95D_H106N_E214V-HaloTag in mammalian cells
129264pCAG-Mac_Q139D_D150N_E258V-HaloTag-STExpress soma-localized Mac_Q139D_D150N_E258V-HaloTag in mammalian cells
129265pTol2-Huc-Positron-STpan neuronal expression of soma-localized Positron in zebrafish
129266pT2-Tbait-UAS-Positron-STUAS-driven expression of Positron in zebrafish
129267pAAV-hsyn-flex-Positron-STCre-dependant expression of soma-localized Positron in neurons

Antibodies from Article