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A Genetic Tool to Track Protein Aggregates and Control Prion Inheritance.

Newby GA, Kiriakov S, Hallacli E, Kayatekin C, Tsvetkov P, Mancuso CP, Bonner JM, Hesse WR, Chakrabortee S, Manogaran AL, Liebman SW, Lindquist S, Khalil AS
Cell. 2017 Nov 2;171(4):966-979.e18. doi: 10.1016/j.cell.2017.09.041. Epub 2017 Oct 19. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
129202pGAN147yeast transcriptional reporter of aggregating proteins (yTRAP) to detect protein aggregation, Gateway backbone
129203pGAN200yTRAP sensor of [PSI+], detects aggregation of Sup35
129204pGAN201yTRAP sensor of [RNQ+], detects aggregation of Rnq1
129249pSK221reverse yTRAP, tetR-responsive Neon reporter
129252pSK260dual yTRAP, NM fusion to aTF1 reporting on [PSI+] state with mKate2 reporter
129268pSK226dual yTRAP, RNQ1 fusion to aTF2 reporting on [RNQ+] state with mNeonGreen reporter
129270pGAN213Control for measuring background levels of yTRAP reporter protein
129272pSK267"ZF only" yTRAP control to control for yTRAP reporter fluorescence changes not dependent on sensed protein
131122pGAN230reverse yTRAP, RNQ1-aTF reporting on tetR-mKate2
131123pGAN101Plasmid for overexpression of prion domains to induce prions
131148pSK247dual yTRAP, Gateway destination vector of aTF2 fusion reporting on mNeonGreen
131149pSK234dual yTRAP, Gateway destination vector of aTF2 fusion reporting on mKate2

Antibodies from Article