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Inhibition of translational initiation by Let-7 MicroRNA in human cells.

Pillai RS, Bhattacharyya SN, Artus CG, Zoller T, Cougot N, Basyuk E, Bertrand E, Filipowicz W
Science. 2005 Sep 2. 309(5740):1573-6. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
115366p CIneo-RLExpression vector to produce humanized Renilla luciferase
115367p CIneo-RL-Let7-perfExpression vector to produce humanized Renilla luciferase with a single perfectly complementary binding site for human let-7 miRNA in the 3'UTR
115368p CIneo-RL-Let7-3xBulgeBExpression vector to produce humanized Renilla luciferase with three partially complementary binding sites for human let-7 miRNA in the 3'UTR
115369p CIneo-RL-Let7-3xBulgeB-mutExpression vector to produce humanized Renilla luciferase with three mutated (seed-sequence mutations) partially complementary binding sites for human let-7 miRNA in the 3'UTR

Antibodies from Article