Tunable molecular tension sensors reveal extension-based control of vinculin loading.
LaCroix AS, Lynch AD, Berginski ME, Hoffman BD
eLife. 2018 Jul 19;7. pii: 33927. doi: 10.7554/eLife.33927.
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PubMed
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
111760 | tCRMod-GGSGGS5 | Expresses soluble Clover-mRuby2 tension sensor module with 5-repeat GGSGGS extensible domain |
111761 | tCRMod-GGSGGS7 | Expresses soluble Clover-mRuby2 tension sensor module with 7-repeat GGSGGS extensible domain |
111762 | tCRMod-GGSGGS9 | Expresses soluble Clover-mRuby2 tension sensor module with 9-repeat GGSGGS extensible domain |
111763 | VinTS-tCRMod-GGSGGS5 | Expresses vinculin tension sensor comprised of Clover-(GGSGGS)5-mRuby2 module |
111764 | VinTS-tCRMod-GGSGGS7 | Expresses vinculin tension sensor comprised of Clover-(GGSGGS)7-mRuby2 module |
111765 | VinTS-tCRMod-GGSGGS9 | Expresses vinculin tension sensor comprised of Clover-(GGSGGS)9-mRuby2 module |