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SypHer3s: a genetically encoded fluorescent ratiometric probe with enhanced brightness and an improved dynamic range.

Ermakova YG, Pak VV, Bogdanova YA, Kotlobay AA, Yampolsky IV, Shokhina AG, Panova AS, Marygin RA, Staroverov DB, Bilan DS, Sies H, Belousov VV
Chem Commun (Camb). 2018 Mar 2. doi: 10.1039/c7cc08740c. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
108118pC1-SypHer3sRatiometric pH probe with enhanced brightness for mammalian cells expression.
108119pC1-SypHer3s-dmitoMitochondrial ratiometric pH probe with enhanced brightness for mammalian cells expression. Probe is localized in mitochondrial matrix.
108120pC1-SypHer3s-IMSMitochondrial ratiometric pH probe with enhanced brightness fused to Smac/DIABLO fragment. Probe is localized in mitochondrial intermembrane space. Mammalian cell expression.

Antibodies from Article