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Ultrafast glutamate sensors resolve high-frequency release at Schaffer collateral synapses.

Helassa N, Durst CD, Coates C, Kerruth S, Arif U, Schulze C, Wiegert JS, Geeves M, Oertner TG, Torok K
Proc Natl Acad Sci U S A. 2018 May 7. pii: 1720648115. doi: 10.1073/pnas.1720648115. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
105832pCMV iGluuFast indicator for extracellular glutamate imaging
105833pCMV iGlufFast indicator for extracellular glutamate imaging
106121pCI syn iGlufFast indicator for synaptic glutamate imaging (synapsin promoter)
106122pCI syn iGluuFast indicator for synaptic glutamate imaging (synapsin promoter)
106123pCI syn iGluSnFRIndicator for glutamate imaging (synapsin promoter)
119828pET41a iGluSnFRProduction of recombinant iGluSnFR, a single-wavelength glutamate indicator, in E. coli
119829pET41a iGlumProduction of recombinant iGluSnFR R24K medium affinity variant, a single-wavelength glutamate indicator, in E. coli
119830pET41a iGluhProduction of recombinant iGluSnFR E25A high affinity variant, a single-wavelength glutamate indicator, in E. coli
119831pET41a iGluSnFR E25RProduction of recombinant iGluSnFR E25R variant, a single-wavelength glutamate indicator, in E. coli
119832pET41a iGlulProduction of recombinant iGluSnFR T92A low affinity variant, a single-wavelength glutamate indicator, in E. coli
119833pET41a iGlufProduction of recombinant iGluSnFR E25D fast variant, a single-wavelength glutamate indicator, in E. coli
119834pET41a iGluuProduction of recombinant iGluSnFR S72T ultrafast variant, a single-wavelength glutamate indicator, in E. coli
119835pET30b GluBP600nProduction of recombinant GluBP600n high affinity protein in E. coli

Antibodies from Article