Ultrafast glutamate sensors resolve high-frequency release at Schaffer collateral synapses.
Helassa N, Durst CD, Coates C, Kerruth S, Arif U, Schulze C, Wiegert JS, Geeves M, Oertner TG, Torok K
Proc Natl Acad Sci U S A. 2018 May 7. pii: 1720648115. doi: 10.1073/pnas.1720648115.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
105832 | pCMV iGluu | Fast indicator for extracellular glutamate imaging |
105833 | pCMV iGluf | Fast indicator for extracellular glutamate imaging |
106121 | pCI syn iGluf | Fast indicator for synaptic glutamate imaging (synapsin promoter) |
106122 | pCI syn iGluu | Fast indicator for synaptic glutamate imaging (synapsin promoter) |
106123 | pCI syn iGluSnFR | Indicator for glutamate imaging (synapsin promoter) |
119828 | pET41a iGluSnFR | Production of recombinant iGluSnFR, a single-wavelength glutamate indicator, in E. coli |
119829 | pET41a iGlum | Production of recombinant iGluSnFR R24K medium affinity variant, a single-wavelength glutamate indicator, in E. coli |
119830 | pET41a iGluh | Production of recombinant iGluSnFR E25A high affinity variant, a single-wavelength glutamate indicator, in E. coli |
119831 | pET41a iGluSnFR E25R | Production of recombinant iGluSnFR E25R variant, a single-wavelength glutamate indicator, in E. coli |
119832 | pET41a iGlul | Production of recombinant iGluSnFR T92A low affinity variant, a single-wavelength glutamate indicator, in E. coli |
119833 | pET41a iGluf | Production of recombinant iGluSnFR E25D fast variant, a single-wavelength glutamate indicator, in E. coli |
119834 | pET41a iGluu | Production of recombinant iGluSnFR S72T ultrafast variant, a single-wavelength glutamate indicator, in E. coli |
119835 | pET30b GluBP600n | Production of recombinant GluBP600n high affinity protein in E. coli |