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Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis.

Martineau M, Somasundaram A, Grimm JB, Gruber TD, Choquet D, Taraska JW, Lavis LD, Perrais D
Nat Commun. 2017 Nov 10;8(1):1412. doi: 10.1038/s41467-017-01752-5. (Link opens in a new window) PubMed

Plasmids from Article

ID Plasmid Purpose
105288VAMP2-SNAPVAMP2 tagged with SNAP
105289VAMP2-pHujiVAMP2 tagged with pH-sensitive red fluorescent protein pHuji
105318VAChT-SNAPVAChT tagged with SNAP
105319VAChT-pHujiVAChT tagged with pH-sensitive red fluorescent protein pHuji
107369VAMP2-pHujiSynaptic vesicle protein VAMP2 fused to the red FP pH sensor pHuji
107371VGluT1-SNAPtagSynaptic vesicle protein VGluT1 fused to the SNAPtag domain
107372VAMP2-SNAPtagSynaptic vesicle protein VAMP2 fused to the SNAPtag domain

Antibodies from Article