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High-performance calcium sensors for imaging activity in neuronal populations and microcompartments.

Dana H, Sun Y, Mohar B, Hulse BK, Kerlin AM, Hasseman JP, Tsegaye G, Tsang A, Wong A, Patel R, Macklin JJ, Chen Y, Konnerth A, Jayaraman V, Looger LL, Schreiter ER, Svoboda K, Kim DS
Nat Methods. 2019 Jul;16(7):649-657. doi: 10.1038/s41592-019-0435-6. Epub 2019 Jun 17. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
104463pGP-CMV-jGCaMP7sMammalian expression of ultrasensitive protein calcium sensor
104483pGP-CMV-jGCaMP7fMammalian expression of ultrasensitive protein calcium sensor
104484pGP-CMV-jGCaMP7bMammalian expression of ultrasensitive protein calcium sensor
104487pGP-AAV-syn-jGCaMP7s-WPREAAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter
104488pGP-AAV-syn-jGCaMP7f-WPREAAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter
104489pGP-AAV-syn-jGCaMP7b-WPREAAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter
104491pGP-AAV-syn-FLEX-jGCaMP7s-WPREAAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre mediated flip-excision switch
104492pGP-AAV-syn-FLEX-jGCaMP7f-WPREAAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre mediated flip-excision switch
104493pGP-AAV-syn-FLEX-jGCaMP7b-WPREAAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre mediated flip-excision switch
104495pGP-AAV-CAG-FLEX-jGCaMP7s-WPREAAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switch
104496pGP-AAV-CAG-FLEX-jGCaMP7f-WPREAAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switch
104497pGP-AAV-CAG-FLEX-jGCaMP7b-WPREAAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switch
105320pGP-CMV-jGCaMP7c variant 1513Mammalian expression of ultrasensitive protein calcium sensor
105321pGP-AAV-syn-jGCaMP7c variant 1513-WPREAAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter
105322pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPREAAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre-dependent expression
105323pGP-AAV-CAG-FLEX-jGCaMP7c variant 1513-WPREAAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre-dependent expression

Antibodies from Article