Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage.
Kulcsar PI, Talas A, Huszar K, Ligeti Z, Toth E, Weinhardt N, Fodor E, Welker E
Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8.
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PubMed
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
80457 | pmCherry_gRNA | gRNA expression vector with mCherry transfection control |
92102 | pX330-Flag-SpCas9-HF1 (without sgRNA) | Expression plasmid for human codon-optimized high-fidelity SpCas9-HF1, px330-like backbone (without U6-sgRNA coding sequence) |
92110 | HeFm1SpCas9 | Expression plasmid for human codon-optimized increased fidelity HeFm1SpCas9 (without U6-sgRNA coding sequence) |
92111 | HeFm2SpCas9 | Expression plasmid for human codon-optimized increased fidelity HeFm2SpCas9 (without U6-sgRNA coding sequence) |
92112 | pX330-HA-dSpCas9 | Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence) |
92113 | pX330-Flag-dSpCas9 | Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence) |
92114 | pX330-Flag-deSpCas9 | Expression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence) |
92115 | pX330-Flag-dSpCas9-HF1 | Expression plasmid for human codon-optimized dead/inactive high-fidelity SpCas9-HF1 (without U6-sgRNA coding sequence) |
92116 | pX330-Flag-dHeFSpCas9 | Expression plasmid for human codon-optimized dead/inactive increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence) |
92117 | pET-deSpCas9-VP64-6xHis | Expression of dead/inactive increased fidelity eSpCas9 (1.1)-VP64-6xHis in bacterial cells |
92118 | pET-dSpCas9-HF1-VP64-6xHis | Expression of dead/inactive increased fidelity SpCas9-HF1-VP64-6xHis in bacterial cells |
92119 | pET-dHeFSpCas9-VP64-6xHis | Expression of dead/inactive increased fidelity HeFSpCas9-VP64-6xHis in bacterial cells |
92120 | U6-sgRNA(MS2)_EF1a-MS2-P65-HSF1 | Expression plasmid for both MS2-P65-HSF1 activator helper complex and sgRNA with two MS2 loops at tetraloop and stemloop 2 contains BsaI sites for insertion of spacer sequences. |
92353 | pX330-Flag-wtSpCas9 (without sgRNA) | Expression plasmid for human codon-optimized wild-type SpCas9 (without U6-sgRNA coding sequence) |
92354 | pX330-Flag-eSpCas9 (without sgRNA) | Expression plasmid for human codon-optimized high-fidelity eSpCas9 (without U6-sgRNA coding sequence) |
92355 | HeFSpCas9 | Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence) |