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Ratiometric Matryoshka biosensors from a nested cassette of green- and orange-emitting fluorescent proteins.

Ast C, Foret J, Oltrogge LM, De Michele R, Kleist TJ, Ho CH, Frommer WB
Nat Commun. 2017 Sep 5;8(1):431. doi: 10.1038/s41467-017-00400-2. (Link opens in a new window) PubMed

Plasmids from Article

ID Plasmid Purpose
100017pET15b-eGO-Matryoshka-LE-LPBacterial expression of fluorescent protein casette - cpEGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkers
100018pET15b-sfGO-Matryoshka-LE-LPBacterial expression of fluorescent protein casette - cpsfGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkers
100019pRSET a MatryoshCaMP6sBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.
100020pRSET a sfMatryoshCaMP6sBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting superfolder cpGFP.
100021pRSET a sfMatryoshCaMP6s-T78HBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference LSSmOrange nested within the reporting superfolder cpGFP-T78H
100022pRSET a sfGCaMP6sBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant.
100023pRSET a sfGCaMP6s-T78HBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant containing T78H mutation
100024pGPTVII-Bar-U-MatryoshCaMP6sPlant expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.
100025pcDNA3.2-DEST-MatryoshCaMP6sMammalian expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.
100922pDRF'- AmTryoshka1;3-LS-F138IYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.
100963pDRF'- AmTryoshka1;3-LS-F138I-T78HYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP
100964pDRF'- AmTryoshka1;3-LS-L255IYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.
100965pDRF'- AmTryoshka1;3-GS-F138IYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.
100966pDRF'- AmTryoshka1;3-GS-L255IYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.
100967pDRF’- AmTryoshka1;3-GSYeast expression of control vector for fluorescent sensors reporting the activity of ammonium transceptors.

Antibodies from Article