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Transmission of innate immune signaling by packaging of cGAMP in viral particles.

Gentili M, Kowal J, Tkach M, Satoh T, Lahaye X, Conrad C, Boyron M, Lombard B, Durand S, Kroemer G, Loew D, Dalod M, Thery C, Manel N
Science. 2015 Sep 11;349(6253):1232-6. doi: 10.1126/science.aab3628. Epub 2015 Jul 30. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
102597IFN-Beta_pGL3IFN-beta promoter driving luciferase. Use in IFN reporter assay.
102602pTRIP-CMV-mTagBFP2-2AControl lentivector. Coexpression of mTagBFP and gene of interest from CMV promoter
102603pTRIP-CMV-mTagBFP2-2A-FLAG-ntcGASLentivector to express Flag-tagged and shRNA-resistant cGAS and mTagBFP
102604pTRIP-CMV-mtagBFP2-2A-FLAG-cGAS E225A/D227ALentivector to express Flag-tagged and catalytically-inactive cGAS and mTagBFP
102605pcDNA3.1-Hygro(+)-PSTCD-cGASMammalian expression of cGAS fused to PSTCD tag
102606pcDNA3.1-Hygro(+)-PSTCD-cGAS-delta DBDMammalian expression of mutant cGAS with deletion of DNA binding domain fused to PSTCD tag
102607pcDNA3.1-Hygro(+)-mscGASMammalian expression of mouse cGAS
102608pMSCV-hygro-STING R232Retroviral vector to expression STING R232
102609pLCRISPR-CMVControl lentivector expressing Cas9 and gRNA scaffold
102610pLCRISPR-CMV-cGAS3CRISPR/Cas9 lentivector targeting human cGAS
102611pTRIP-CMV-Puro-2AControl lentivector expressing Puro resistance and gene of interest from CMV promoter
102612pTRIP-CMV-Puro-2A-cGASLentivector expressing cGAS

Antibodies from Article