Transmission of innate immune signaling by packaging of cGAMP in viral particles.
Gentili M, Kowal J, Tkach M, Satoh T, Lahaye X, Conrad C, Boyron M, Lombard B, Durand S, Kroemer G, Loew D, Dalod M, Thery C, Manel N
Science. 2015 Sep 11;349(6253):1232-6. doi: 10.1126/science.aab3628. Epub 2015 Jul 30.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
102597 | IFN-Beta_pGL3 | IFN-beta promoter driving luciferase. Use in IFN reporter assay. |
102602 | pTRIP-CMV-mTagBFP2-2A | Control lentivector. Coexpression of mTagBFP and gene of interest from CMV promoter |
102603 | pTRIP-CMV-mTagBFP2-2A-FLAG-ntcGAS | Lentivector to express Flag-tagged and shRNA-resistant cGAS and mTagBFP |
102604 | pTRIP-CMV-mtagBFP2-2A-FLAG-cGAS E225A/D227A | Lentivector to express Flag-tagged and catalytically-inactive cGAS and mTagBFP |
102605 | pcDNA3.1-Hygro(+)-PSTCD-cGAS | Mammalian expression of cGAS fused to PSTCD tag |
102606 | pcDNA3.1-Hygro(+)-PSTCD-cGAS-delta DBD | Mammalian expression of mutant cGAS with deletion of DNA binding domain fused to PSTCD tag |
102607 | pcDNA3.1-Hygro(+)-mscGAS | Mammalian expression of mouse cGAS |
102608 | pMSCV-hygro-STING R232 | Retroviral vector to expression STING R232 |
102609 | pLCRISPR-CMV | Control lentivector expressing Cas9 and gRNA scaffold |
102610 | pLCRISPR-CMV-cGAS3 | CRISPR/Cas9 lentivector targeting human cGAS |
102611 | pTRIP-CMV-Puro-2A | Control lentivector expressing Puro resistance and gene of interest from CMV promoter |
102612 | pTRIP-CMV-Puro-2A-cGAS | Lentivector expressing cGAS |