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Rapidly inducible Cas9 and DSB-ddPCR to probe editing kinetics.

Rose JC, Stephany JJ, Valente WJ, Trevillian BM, Dang HV, Bielas JH, Maly DJ, Fowler DM
Nat Methods. 2017 Jul 24. doi: 10.1038/nmeth.4368. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
100550ciCas9_pcDNA5Expresses ciCas9 in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.
100551ciCas9(L22)_pcDNA5Expresses ciCas9(L22) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.
100552ciCas9(F22)_pcDNA5Expresses ciCas9(F22) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.
100553e-ciCas9_pcDNA5Expresses enhanced specificity ciCas9 (e-ciCas9) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.
100554AAVS1_sgRNAExpresses AAVS1 sgRNA. Target sequence: GGGGCCACTAGGGACAGGAT
100555EMX1_sgRNAExpresses EMX1 sgRNA. Target sequence: GAGTCCGAGCAGAAGAAGAA
100556MYC sgRNA4Expresses MYC sgRNA4. Target sequence: GTAATTCCAGCGAGAGGCAG
100557MYC sgRNA5Expresses MYC sgRNA5. Target sequence GAGAGGCAGAGGGAGCGAGC
100558EMX1*_sgRNAExpresses EMX1* sgRNA. Target sequence: (G)GAGTCCGAGCAGAAGAAGAA. Same target sequence as #100555, except with an additional 5' G.

Antibodies from Article