Resolving systematic errors in widely used enhancer activity assays in human cells.
Muerdter F, Boryn LM, Woodfin AR, Neumayr C, Rath M, Zabidi MA, Pagani M, Haberle V, Kazmar T, Catarino RR, Schernhuber K, Arnold CD, Stark A
Nat Methods. 2017 Dec 11. pii: nmeth.4534. doi: 10.1038/nmeth.4534. PubMed Article
Muerdter F, Boryn LM, Woodfin AR, Neumayr C, Rath M, Zabidi MA, Pagani M, Haberle V, Kazmar T, Catarino RR, Schernhuber K, Arnold CD, Stark A
Nat Methods. 2017 Dec 11. pii: nmeth.4534. doi: 10.1038/nmeth.4534. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 99291 | hSTARR-seq_mCP vector | Vector to measure enhancer activity of candidates from a DNA library by STARR-seq. |
| 99292 | hSTARR-seq_SCP1 vector | Vector to measure enhancer activity of candidates from a DNA library by STARR-seq. |
| 99293 | hSTARR-seq_TTF2 vector | Vector to measure enhancer activity of candidates from a DNA library by STARR-seq. |
| 99294 | hSTARR-seq_SULT1C vector | Vector to measure enhancer activity of candidates from a DNA library by STARR-seq. |
| 99295 | hSTARR-seq_OCT4 3UTR vector | Vector to measure enhancer activity of candidates from a DNA library by STARR-seq. |
| 99296 | hSTARR-seq_ORI vector | Vector to measure enhancer activity of candidates from a DNA library by STARR-seq. |
| 99297 | STARR-seq luciferase validation vector_ORI_empty | Luciferase validation vector; empty vector without insert to determine baseline |
| 99298 | STARR-seq luciferase validation vector_mP_empty | Luciferase validation vector; empty vector without insert to determine baseline |
| 99299 | STARR-seq luciferase validation vector_SCP1_empty | Luciferase validation vector; empty vector without insert to determine baseline |
| 99300 | STARR-seq luciferase validation vector_ORI_AGAP1 | Luciferase validation vector with AGAP1 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity |
| 99301 | STARR-seq luciferase validation vector_mP_AGAP1 | Luciferase validation vector with AGAP1 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity |
| 99302 | STARR-seq luciferase validation vector_SCP1_AGAP1 | Luciferase validation vector with AGAP1 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity |
| 99303 | STARR-seq luciferase validation vector_ORI_GTSE1 | Luciferase validation vector with GTSE1 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity |
| 99304 | STARR-seq luciferase validation vector_mP_GTSE1 | Luciferase validation vector with GTSE1 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity |
| 99305 | STARR-seq luciferase validation vector_SCP1_GTSE1 | Luciferase validation vector with GTSE1 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity |
| 99306 | STARR-seq luciferase validation vector_ORI_IGF1R | Luciferase validation vector with IGF1R enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity |
| 99307 | STARR-seq luciferase validation vector_mP_IGF1R | Luciferase validation vector with IGF1R enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity |
| 99308 | STARR-seq luciferase validation vector_SCP1_IGF1R | Luciferase validation vector with IGF1R enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity |
| 99309 | STARR-seq luciferase validation vector_ORI_SV40 | Luciferase validation vector with SV40 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity |
| 99310 | STARR-seq luciferase validation vector_mP_SV40 | Luciferase validation vector with SV40 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity |
| 99311 | STARR-seq luciferase validation vector_SCP1_SV40 | Luciferase validation vector with SV40 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity |
| 99312 | STARR-seq luciferase validation vector_ORI_CMV | Luciferase validation vector with CMV enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity |
| 99313 | STARR-seq luciferase validation vector_mP_CMV | Luciferase validation vector with CMV enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity |
| 99314 | STARR-seq luciferase validation vector_SCP1_CMV | Luciferase validation vector with CMV enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity |
| 99315 | STARR-seq luciferase validation vector_ORI_neg.cont | Negative control for luciferase validation vectors |
| 99316 | hSTARR-seq_SCP1 vector_blocking 1 | Vector to measure enhancer activity of candidates from a DNA library by STARR-seq. |
| 99317 | hSTARR-seq_SCP1 vector_blocking 2 | Vector to measure enhancer activity of candidates from a DNA library by STARR-seq. |
| 99318 | hSTARR-seq_SCP1 vector_blocking 3 | Vector to measure enhancer activity of candidates from a DNA library by STARR-seq. |
| 99319 | hSTARR-seq_SCP1 vector_blocking 4 | Vector to measure enhancer activity of candidates from a DNA library by STARR-seq. |
| 99320 | STARR-seq luciferase INF enhancer vector_ORI_IFI27 | Luciferase validation vector with IFI27 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity |
| 99321 | STARR-seq luciferase INF enhancer vector_ORI_IFIT2 | Luciferase validation vector with IFIT2 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity |
| 99322 | STARR-seq luciferase INF enhancer vector_ORI_ISG15 | Luciferase validation vector with ISG15 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity |
| 99323 | STARR-seq luciferase INF enhancer vector_ORI_MX1 | Luciferase validation vector with MX1 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity |
| 99324 | STARR-seq luciferase INF enhancer vector_ORI_OAS3 | Luciferase validation vector with OAS3 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity |