Nucleic acid detection with CRISPR-Cas13a/C2c2.
Gootenberg JS, Abudayyeh OO, Lee JW, Essletzbichler P, Dy AJ, Joung J, Verdine V, Donghia N, Daringer NM, Freije CA, Myhrvold C, Bhattacharyya RP, Livny J, Regev A, Koonin EV, Hung DT, Sabeti PC, Collins JJ, Zhang F
Science. 2017 Apr 13. pii: eaam9321. doi: 10.1126/science.aam9321.
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PubMed
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Article
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
90097 | pC013 - Twinstrep-SUMO-huLwCas13a | Twinstrep-SUMO-huLwCas13a for recombinant protein bacterial expression. Insert is human codon optimized but expresses well in bacteria. |
91900 | pC009 LshCas13a locus with targeting spacer | LshCas13a locus into pACYC184 with targeting spacer |
91901 | pC010 LshCas13a locus with nontargeting spacer | LshCas13a locus into pACYC184 with nontargeting spacer |
91903 | pC011 LwCas13a locus with targeting spacer | LwCas13a locus into pACYC184 with targeting spacer |
91904 | pC012 LwCas13a locus with nontargeting spacer | LwCas13a locus into pACYC184 with nontargeting spacer |