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Nucleic acid detection with CRISPR-Cas13a/C2c2.

Gootenberg JS, Abudayyeh OO, Lee JW, Essletzbichler P, Dy AJ, Joung J, Verdine V, Donghia N, Daringer NM, Freije CA, Myhrvold C, Bhattacharyya RP, Livny J, Regev A, Koonin EV, Hung DT, Sabeti PC, Collins JJ, Zhang F
Science. 2017 Apr 13. pii: eaam9321. doi: 10.1126/science.aam9321. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
90097pC013 - Twinstrep-SUMO-huLwCas13aTwinstrep-SUMO-huLwCas13a for recombinant protein bacterial expression. Insert is human codon optimized but expresses well in bacteria.
91900pC009 LshCas13a locus with targeting spacerLshCas13a locus into pACYC184 with targeting spacer
91901pC010 LshCas13a locus with nontargeting spacerLshCas13a locus into pACYC184 with nontargeting spacer
91903pC011 LwCas13a locus with targeting spacerLwCas13a locus into pACYC184 with targeting spacer
91904pC012 LwCas13a locus with nontargeting spacerLwCas13a locus into pACYC184 with nontargeting spacer

Antibodies from Article