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Large-scale design of robust genetic circuits with multiple inputs and outputs for mammalian cells.

Weinberg BH, Pham NT, Caraballo LD, Lozanoski T, Engel A, Bhatia S, Wong WW
Nat Biotechnol. 2017 Mar 27. doi: 10.1038/nbt.3805. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
87549pBW1997_pExpr-hU6-CRISPR-DECODERExpresses NGN2, MIAT, ACTC1, and TTN gRNAs in response to combinations of Cre and Flp recombinases
87551pBW842_pCAG-pExpr-FP-DECODERExpresses BFP, GFP, iRFP, and mRuby in response to combinations of Cre and Flp recombinases
87552pBW820_pCAG-pExpr-FULL-ADDEREncodes full adder logic in response to Cre, Flp and VCre recombinases
87554pBW829_pCAG-pExpr-LUTEncodes Boolean Look-up Table logic in response to Cre, Flp, PhiC31, Vika, B3, and Bxb1 recombinases
87559pBW2286_pCAG-PV1-FlpO-N396-L1-ABI-NLS-BGHpAExpresses aa1-396 of Flp recombinase fused to ABI CID
87560pBW2287_pCAG-PV1-PYL-L1-FlpO-397C-NLS-BGHpAExpresses aa397-423 of Flp recombinase fused to PYL CID
87561pBW701_pCAG-PV1Simple PV1
87562pBW702_pCAG-PV2Simple PV2
87563pBW703_pCAG-PV3Simple PV3
87564pBW704_pCAG-PV4Simple PV4
87565pBW751_pCAG-PV5Simple PV5
87566pBW752_pCAG-PV6Simple PV6
87567pBW753_pCAG-PV7Simple PV7
87568pBW754_pCAG-PV8Simple PV8
87569pBW705_pCAG-PV1-FRT-lox2272-STOPPV1 for 2-input, 2-output circuit library
87570pBW706_pCAG-PV2-lox2272-GFPPV2 for 2-input, 2-output circuit library
87571pBW707_pCAG-PV3-FRT-loxP-mCherryPV3 for 2-input, 2-output circuit library
87572pBW1944_ hU6-PV4-loxP-BbsI-ENTRY-gRNAPV4 gRNA entry vector for CRISPR decoder
87574pBW768_pCAG-PV1-FRT-lox2272-BFP-BGHpAPV1 for 2-input, 4-output decoder circuit
87575pBW759_pCAG-PV2-lox2272-GFP-BGHpAPV2 for 2-input, 4-output decoder circuit and 3-input, 2-output Full Adder
87576pBW769_pCAG-PV3-FRT-loxP-iRFP720-BGHpAPV3 for 2-input, 4-output decoder circuit
87577pBW770_pCAG-PV4-loxP-mRuby2-BGHpAPV4 for 2-input, 4-output decoder circuit
87578pBW700_pCAG-pDest-U1-UXpCAG destination vector (U1-UX)
87579pBW1716_hU6-pDest-U1-UXhU6 destination vector
87580pBW758_pCAG-PV1-VloxP-FRT-lox2272-NeoR-BGHpAPV1 for 3-input, 2-output Full Adder
87582pBW806_pCAG-PV3-FRT-loxP-GFP-BGHpAPV3 for 3-input, 2-output Full Adder
87583pBW807_pCAG-PV4-loxP-mCherry-BGHpAPV4 for 3-input, 2-output Full Adder
87584pBW808_pCAG-PV5-VloxP-loxN-F3-GFP-BGHpAPV5 for 3-input, 2-output Full Adder
87585pBW809_pCAG-PV6-F3-mCherry-BGHpAPV6 for 3-input, 2-output Full Adder
87586pBW810_pCAG-PV7-loxN-F14-mCherry-BGHpAPV7 for 3-input, 2-output Full Adder
87587pBW811_pCAG-PV8-F14-GFP-T2A-mCherry-BGHpAPV8 for 3-input, 2-output Full Adder
89053pBW1720_hU6-PV1-FRT-lox2272-BbsI-ENTRY-gRNAPV1 gRNA entry vector for CRISPR decoder
89055pBW1942_ hU6-PV2-lox2272-BbsI-ENTRY-gRNAPV2 gRNA entry vector for CRISPR decoder
89056pBW1943_ hU6-PV3-FRT-loxP-BbsI-ENTRY-gRNAPV3 gRNA entry vector for CRISPR decoder
89057pBW708_pCAG-PV4-loxP-GFP-T2A-mCherryPV4 for 2-input, 2-output circuit library
89507pBW755_pCAG-pDest-U5-UXpCAG destination vector (U5-UX)
89573pCAG-iCreExpresses iCre under pCAG
89574pCAG-FlpOExpresses FlpO under pCAG
89575pCAG-VCreExpresses VCre under pCAG
89684pCAG-EGFPpCAG promoter driving EGFP
89685pCAG-mtagBFPpCAG promoter driving mtagBFP
89686pCAG-mRuby2pCAG promoter driving mRuby2
89687pCAG-iRFP720pCAG promoter driving iRFP720
89688pCAG-LSS-mOrangepCAG promoter driving LSS-mOrange
89689pCAG-FALSEpCAG promoter driving no CDS

Antibodies from Article