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A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia.

Tzelepis K, Koike-Yusa H, De Braekeleer E, Li Y, Metzakopian E, Dovey OM, Mupo A, Grinkevich V, Li M, Mazan M, Gozdecka M, Ohnishi S, Cooper J, Patel M, McKerrell T, Chen B, Domingues AF, Gallipoli P, Teichmann S, Ponstingl H, McDermott U, Saez-Rodriguez J, Huntly BJ, Iorio F, Pina C, Vassiliou GS, Yusa K
Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
67974pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-WCRISPR gRNA expression vector with an improved scaffold and puro/BFP markers
67975pKLV2-U6gRNA5(BbsI)-PGKpuro2AZsG-WCRISPR gRNA expression vector with an improved scaffold and puro/ZsG markers
67976pKLV2-U6gRNA5(BbsI)-PGKpuro2AmAG-WCRISPR gRNA expression vector with an improved scaffold and puro/mAG markers
67977pKLV2-U6gRNA5(BbsI)-PGKpuro2AmCherry-WCRISPR gRNA expression vector with an improved scaffold and puro/mCherry markers
67978pKLV2-EF1a-BsdCas9-WLentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the N-terminus
67979pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-WCas9 activity reporter (control) with BFP and GFP.
67980pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-WCas9 activity reporter with BFP and GFP.
67981pKLV2-U6gRNA5(Empty)-PGKmCherry2AGFP-WCas9 activity reporter (control) with mCherry and GFP
67982pKLV2-U6gRNA5(gGFP)-PGKmCherry2AGFP-WCas9 activity reporter with mCherry and GFP
67983pKLV2-U6gRNA5(Empty)-PGKGFP2ABFP-WCas9 activity reporter (control) with GFP and BFP
67984pKLV2-U6gRNA5(gBFP)-PGKGFP2ABFP-WCas9 activity reporter with GFP and BFP
67985pKLV2-U6gRNA5(Empty)-PGKmCherry2ABFP-WCas9 activity reporter (control) with mCherry and BFP
67986pKLV2-U6gRNA5(gBFP)-PGKmCherry2ABFP-WCas9 activity reporter with mCherry and BFP
67987pRosa26-EF1a-hCas9IRESneoMouse Rosa26 targeting vector carrying the EF1a-hCas9-IRES-neo cassette
67989Human genome-wide lentiviral CRISPR gRNA library version 1Human genome-wide CRISPR guide RNA library V1 (pKLV2-U6gRNA5(HumanV1)-PGKpuro2ABFP-W)
67990pKLV2-U6gRNA3(BbsI)-PGKpuro2ABFPCRISPR gRNA expression vector with the conventional scaffold and puro/BFP markers without WPRE
67991pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFPCRISPR gRNA expression vector with an improved l scaffold and puro/BFP markers without WPRE
68343pKLV2-EF1a-Cas9Bsd-WLentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the C-terminus
72666pKLV2.2-h7SKgRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-WLentiviral dual CRISPR gRNA expression vector (human 7SK and human U6 promoters)
72667pKLV2.2-mU6gRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-WLentiviral dual CRISPR gRNA expression vector (mouse U6 and human U6 promoters)

Antibodies from Article