cAMPr: A single-wavelength fluorescent sensor for cyclic AMP.
Hackley CR, Mazzoni EO, Blau J
Sci Signal. 2018 Mar 6;11(520). pii: 11/520/eaah3738. doi: 10.1126/scisignal.aah3738.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
84932 | pUASTattb-cAMPr Y205A, R626A | cAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R626A should decrease affinity for cAMP without affecting specifity. |
84933 | pUASTattb-cAMPr Y205A, R739A | cAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R739A should increase affinity for cAMP without affecting specificity. |
99143 | p2lox-cAMPr | Expresses cAMPr in mammalian cells |
102437 | p2lox-cAMPr R626A | cAMPr with a point mutation R626A which theoretically decreases its affinity for cAMP |
102438 | p2lox-cAMPr Y205A | cAMPr with a point mutation Y205A which should render PKA-C catalytically dead |
102439 | p2lox-N-(His-6X)-cAMPr | cAMPr with a directly fused N-terminal 6x His tag |
102618 | p2lox-cAMPr R739A | cAMPr with a point mutation R739A which theoretically increases its affinity for cAMP |