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cAMPr: A single-wavelength fluorescent sensor for cyclic AMP.

Hackley CR, Mazzoni EO, Blau J
Sci Signal. 2018 Mar 6;11(520). pii: 11/520/eaah3738. doi: 10.1126/scisignal.aah3738. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
84932pUASTattb-cAMPr Y205A, R626AcAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R626A should decrease affinity for cAMP without affecting specifity.
84933pUASTattb-cAMPr Y205A, R739AcAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R739A should increase affinity for cAMP without affecting specificity.
99143p2lox-cAMPrExpresses cAMPr in mammalian cells
102437p2lox-cAMPr R626AcAMPr with a point mutation R626A which theoretically decreases its affinity for cAMP
102438p2lox-cAMPr Y205AcAMPr with a point mutation Y205A which should render PKA-C catalytically dead
102439p2lox-N-(His-6X)-cAMPrcAMPr with a directly fused N-terminal 6x His tag
102618p2lox-cAMPr R739AcAMPr with a point mutation R739A which theoretically increases its affinity for cAMP

Antibodies from Article