DIGIT Is a Conserved Long Noncoding RNA that Regulates GSC Expression to Control Definitive Endoderm Differentiation of Embryonic Stem Cells.
Daneshvar K, Pondick JV, Kim BM, Zhou C, York SR, Macklin JA, Abualteen A, Tan B, Sigova AA, Marcho C, Tremblay KD, Mager J, Choi MY, Mullen AC
Cell Rep. 2016 Oct 4;17(2):353-365. doi: 10.1016/j.celrep.2016.09.017.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
82695 | pgRGFP | Expresses gRNA of interest under U6 promoter (cloning by BpiI) and GFP under PGK promoter. |
82696 | pCRE-iRFP670 | Expresses CRE and iRFP670 under two separate PGK promoters. |
82697 | pAAVS1-shRNA | For targeting shRNA construct into human AAVS1 locus, using genome editing. Expresses shRNA of interest (cloning: EcoRI and AgeI) under U6 promoter, flanked by AAVS1 homology arms. |