Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems.
Nishida K, Arazoe T, Yachie N, Banno S, Kakimoto M, Tabata M, Mochizuki M, Miyabe A, Araki M, Hara KY, Shimatani Z, Kondo A
Science. 2016 Aug 4. pii: aaf8729.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 79612 | pRS315e_pGal-PmCDA1 | Expresses PmCDA1 in yeast cells |
| 79613 | pRS315e_pGal-dCas9-SH3 | Expresses dCas9-SH3 in yeast cells |
| 79614 | pRS315e_pGal-dCas9-SH3-pGal-PmCDA1-SHL | Expresses dCas9-SH3 and PmCDA1-SHL in yeast cells |
| 79615 | pRS315e_pGal-dCas9-PmCDA1 | Expresses dCas9-PmCDA1 in yeast cells |
| 79616 | pRS415_pGal-nCas9(H840A) | Expresses nCas9(H840A) in yeast cells |
| 79617 | pRS315e_pGal-nCas9(D10A)-PmCDA1 | Expresses nCas9(D10A)-PmCDA1 in yeast cells |
| 79618 | pRS415_pGal-nCas9(D10A) | Expresses nCas9(D10A) in yeast cells |
| 79619 | pcDNA3.1_pCMV-nCas-PmCDA1 pH1-gRNA(HPRT) | Expresses nCas9-PmCDA1 and gRNA(HPRT) in mammalian cells |
| 79620 | pcDNA3.1_pCMV-nCas-PmCDA1-ugi pH1-gRNA(HPRT) | Expresses nCas9-PmCDA1-UGI and gRNA(HPRT) in mammalian cells |
| 79621 | pcDNA3.1_pCMV-dCas-PmCDA1 pH1-gRNA(HPRT) | Expresses dCas9-PmCDA1 and gRNA(HPRT) in mammalian cells |