Addgene: Golden Gate Assembly of CRISPR gRNA expression array for simultaneously targeting multiple genes.

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Yonglun Luo
Vad-Nielsen et al

Golden Gate Assembly of CRISPR gRNA expression array for simultaneously targeting multiple genes.
Vad-Nielsen J, Lin L, Bolund L, Nielsen AL, Luo Y
Cell Mol Life Sci. 2016 May 13. PubMed

Plasmids from Article

ID	Plasmid	Purpose
80784	pMA-SpCas9-g1	BsaI-based cloning of SpCas9 gRNA guide sequence, position 1/11/21 in the array
80785	pMA-SpCas9-g2	BsaI-based cloning of SpCas9 gRNA guide sequence, position 2/12/22 in the array
80786	pMA-SpCas9-g3	BsaI-based cloning of SpCas9 gRNA guide sequence, position 3/13/23 in the array
80787	pMA-SpCas9-g4	BsaI-based cloning of SpCas9 gRNA guide sequence, position 4/14/24 in the array
80788	pMA-SpCas9-g5	BsaI-based cloning of SpCas9 gRNA guide sequence, position 5/15/25 in the array
80789	pMA-SpCas9-g6	BsaI-based cloning of SpCas9 gRNA guide sequence, position 6/16/26 in the array
80790	pMA-SpCas9-g7	BsaI-based cloning of SpCas9 gRNA guide sequence, position 7/17/27 in the array
80791	pMA-SpCas9-g8	BsaI-based cloning of SpCas9 gRNA guide sequence, position 8/18/28 in the array
80792	pMA-SpCas9-g9	BsaI-based cloning of SpCas9 gRNA guide sequence, position 9/19/29 in the array
80793	pMA-SpCas9-g10	BsaI-based cloning of SpCas9 gRNA guide sequence, position 10/20/30 in the array
80794	pMA-MsgRNA-EGFP	For insertion of gRNA array containing 11-30 gRNA modules

Antibodies from Article