Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library.
Koike-Yusa H, Li Y, Tan EP, Velasco-Herrera Mdel C, Yusa K
Nat Biotechnol. 2014 Mar;32(3):267-73. doi: 10.1038/nbt.2800. Epub 2013 Dec 23.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
50946 | pKLV-U6gRNA(BbsI)-PGKpuro2ABFP | An empty gRNA expression vector |