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Development of a Reagentless Biosensor for Inorganic Phosphate, Applicable over a Wide Concentration Range.

Solscheid C, Kunzelmann S, Davis CT, Hunter JL, Nofer A, Webb MR
Biochemistry. 2015 Aug 18;54(32):5054-62. doi: 10.1021/acs.biochem.5b00449. Epub 2015 Aug 4. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
78198pET22b_PstS_1Expression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor
78199pET22b_PstS_2Expression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor
78200pET22b_PstS_3Expression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensor

Antibodies from Article