Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow.
Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T
Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 75381 | pHAGE-TO-dCas9 | dCas9 |
| 75383 | pHAGE-TO-MCP-3XBFPnls | MCP-3XBFPnls |
| 75384 | pHAGE-EFS-MCP-3XBFPnls | MCP-3XBFPnls |
| 75385 | pHAGE-EFS-PCP-3XGFPnls | PCP-3XGFPnls |
| 75386 | pHAGE-EFS-PCP-3XNeon-nls | PCP-3XNeon-nls |
| 75387 | pHAGE-EFS-N22p-3XRFPnls | N22p-3XRFPnls |
| 75388 | pLH-sgRNA1 | sgRNA1 |
| 75389 | pLH-sgRNA1-2XMS2 | sgRNA1-2XMS2 |
| 75390 | pLH-sgRNA1-2XPP7 | sgRNA1-2XPP7 |
| 75391 | pLH-sgRNA1-2XboxB | sgRNA1-2XboxB |
| 75392 | pLH-sgRNA1-MS2-PP7 | sgRNA1-MS2-PP7 |
| 75393 | pLH-sgRNA1-PP7-boxB | sgRNA1-PP7-boxB |
| 75396 | pLH-sgRNA1-boxB-MS2 | sgRNA1-boxB-MS2 |
| 75397 | pLH-sgRNA1-boxB-MS2-PP7 | sgRNA1-boxB-MS2-PP7 |
| 75398 | pCRISPRainbow-DONOR1 | CRISPRainbow-DONOR1 |