Efficient gene editing in Neurospora crassa with CRISPR technology.
Matsu-Ura T, Baek M, Kwon J, Hong C
Fungal Biol Biotechnol. 2015 Jul 15;2:4. doi: 10.1186/s40694-015-0015-1. eCollection 2015.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
68059 | p415-PtrpC-Cas9-TtrpC-CYC1t | Express humanized Cas9 under the control of trpC promoter and terminator from A. nidulans. |
68060 | p426-SNR52p-gRNA.csr-1.Y-SUP4t | gRNA for csr-1 locus in N. crassa |
89692 | p426-SNR52p-gRNA.clr-2.Y-SUP4t | gRNA for clr-2 locus |
89693 | M249-5'csr-1-Pgsy-1-luciferase-Bar-3'csr-1 | Luciferase reporter plasmid targeted at csr-1 locus |