pAP546-2
(Plasmid
#99476)
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Purpose3XFlag::mNeptune::Myc
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 99476 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC19
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name3XFlag::mNeptune::Myc
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SpeciesC. elegans (nematode)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer M13pUC-Fwd
- 3′ sequencing primer M13pUC-Rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAP546-2 was a gift from Geraldine Seydoux (Addgene plasmid # 99476 ; http://n2t.net/addgene:99476 ; RRID:Addgene_99476) -
For your References section:
Precision genome editing using CRISPR-Cas9 and linear repair templates in C. elegans. Paix A, Folkmann A, Seydoux G. Methods. 2017 May 15;121-122:86-93. doi: 10.1016/j.ymeth.2017.03.023. Epub 2017 Apr 7. 10.1016/j.ymeth.2017.03.023 PubMed 28392263