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PurposeHomologous recombination donor for pDRR. Expresses mCherry after repait by HR.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 98896 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCAGGS
- Backbone size w/o insert (bp) 3050
- Total vector size (bp) 6435
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Modifications to backboneDeletion of CBA promoter
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Vector typeMammalian Expression, Synthetic Biology
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Selectable markersBFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNeomycin-T2A-mCherry-GFP
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SpeciesSynthetic
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Insert Size (bp)2290
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAGGS DRR mCherry Donor EF1a BFP was a gift from Jan Karlseder (Addgene plasmid # 98896 ; http://n2t.net/addgene:98896 ; RRID:Addgene_98896) -
For your References section:
Regulation of DNA repair pathway choice in S and G2 phases by the NHEJ inhibitor CYREN. Arnoult N, Correia A, Ma J, Merlo A, Garcia-Gomez S, Maric M, Tognetti M, Benner CW, Boulton SJ, Saghatelian A, Karlseder J. Nature. 2017 Sep 20;549(7673):548-552. doi: 10.1038/nature24023. 10.1038/nature24023 PubMed 28959974