pTNFR1-tdEOS
(Plasmid #98273)

• Purpose: mammalian expression of TNFR1-tdEOS
• Depositing Lab: Mike Heilemann
• Publication: Heidbreder et al Biochim Biophys Acta. 2012 Oct;1823(10):1984-9. doi: 10.1016/j.bbamcr.2012.06.026. Epub 2012 Jun 27.

Backbone

• Vector backbone: pcDNA3-Flag1-tdEosFP
• Backbone manufacturer: MoBiTec
• Vector type: Mammalian Expression
• Selectable markers: tdEos

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TNFR1
• Alt name: TNFRSF1A
• Species: H. sapiens (human)
• Insert Size (bp): 1392
• Entrez Gene: TNFRSF1A (a.k.a. CD120a, FPF, TBP1, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR1, TNFR55, TNFR60, p55, p55-R, p60)
• Promoter: CMV
• Tags / Fusion Proteins:
  • Flag (N terminal on backbone)
  • tdEos (C terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F

Resource Information

• Supplemental Documents:
  • TNFR1-EosFP.gb
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

fusion protein: TNFR1-tdEosfp