CTL2 (M2225R)
(Plasmid
#96952)
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PurposeExpresses C-Terminal segment of C. elegans Piezo1 with M2225R mutation in E. coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 96952 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET21b
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5300
- Total vector size (bp) 6222
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCTL2 (M222R) of PIEZO1
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Alt nameC Terminal Loop 2 (M2225R) of PIEZO1
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Alt namePIEZO1
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SpeciesC. elegans (nematode)
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GenBank ID392900032
- Promoter T7 Promoter
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Tag
/ Fusion Protein
- 6x Histidine Tag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (destroyed during cloning)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7 Promoter
- 3′ sequencing primer T7 Terminator (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Note: M2225R mutation refers to human mutation of gene. M2092R is mutation in C. elegans.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CTL2 (M2225R) was a gift from Douglas Rees (Addgene plasmid # 96952 ; http://n2t.net/addgene:96952 ; RRID:Addgene_96952) -
For your References section:
The structure of a conserved piezo channel domain reveals a topologically distinct beta sandwich fold. Kamajaya A, Kaiser JT, Lee J, Reid M, Rees DC. Structure. 2014 Oct 7;22(10):1520-7. doi: 10.1016/j.str.2014.08.009. Epub 2014 Sep 18. 10.1016/j.str.2014.08.009 PubMed 25242456