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PurposeTo label mature excitatory iPSC-derived human neurons
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 96942 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLenti
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Vector typeLentiviral
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Selectable markersZeo marker is outside the LTRs and will not be packaged into virus.
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namefar-red fluorescent protein
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Alt namemKate2
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MutationE87D (please see depositor comments below)
- Promoter CaMKIIa
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CCGGTGCCACCATGGTGA (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that mutation E87D in mKate2 was found during Addgene's quality control process. The depositor noted that this mutation does NOT affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti_CamKIIa_mKate2 was a gift from Ricardo Dolmetsch & Oleksandr Shcheglovitov (Addgene plasmid # 96942 ; http://n2t.net/addgene:96942 ; RRID:Addgene_96942) -
For your References section:
SHANK3 and IGF1 restore synaptic deficits in neurons from 22q13 deletion syndrome patients. Shcheglovitov A, Shcheglovitova O, Yazawa M, Portmann T, Shu R, Sebastiano V, Krawisz A, Froehlich W, Bernstein JA, Hallmayer JF, Dolmetsch RE. Nature. 2013 Nov 14;503(7475):267-71. doi: 10.1038/nature12618. Epub 2013 Oct 16. 10.1038/nature12618 PubMed 24132240