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Addgene

YopH-pNIC-Bsa4
(Plasmid #92063)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 92063 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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Backbone

  • Vector backbone
    pNIC28-Bsa4
  • Backbone size w/o insert (bp) 5356
  • Total vector size (bp) 6277
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    YopH
  • Species
    Yersinia pestis
  • Insert Size (bp)
    921
  • Mutation
    Codon optimized E. coli
  • Tag / Fusion Protein
    • 6x His Tag (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer TACTTCCAATCCATG
  • 3′ sequencing primer TATCCACCTTTACTG
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YopH-pNIC-Bsa4 was a gift from Josh Beckham (Addgene plasmid # 92063 ; http://n2t.net/addgene:92063 ; RRID:Addgene_92063)