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PurposeExpresses negative-feedback dLwCas13a for mammalian RNA targeting/imaging
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 91905 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCAGEN
- Backbone size w/o insert (bp) 5876
- Total vector size (bp) 10622
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedLwCas13a
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Alt namedead LwaCas13a
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Alt namedead LwC2c2
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Alt namedCas13a
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SpeciesH. sapiens (human); Leptotrichia wadei
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Insert Size (bp)4806
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MutationR474A and R1046A
- Promoter CAG
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Tags
/ Fusion Proteins
- eGFP, ZF CCR5TC, KRAB-A, NLS (C terminal on insert)
- NLS (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pCAG-F (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pC015 - dLwCas13a-NF was a gift from Feng Zhang (Addgene plasmid # 91905 ; http://n2t.net/addgene:91905 ; RRID:Addgene_91905) -
For your References section:
RNA targeting with CRISPR-Cas13. Abudayyeh OO, Gootenberg JS, Essletzbichler P, Han S, Joung J, Belanto JJ, Verdine V, Cox DBT, Kellner MJ, Regev A, Lander ES, Voytas DF, Ting AY, Zhang F. Nature. 2017 Oct 4. doi: 10.1038/nature24049. 10.1038/nature24049 PubMed 28976959