pLIX402 caPHF2
(Plasmid
#91701)
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Purposeinducible lentiviral expression of constitutively active PHF2
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 91701 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLIX402
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Backbone manufacturerDavid Root (Addgene plasmid # 41394)
- Backbone size w/o insert (bp) 9394
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePHF2
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SpeciesH. sapiens (human)
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MutationcaPHF2; S757D, S899D, S954D, S1056D
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GenBank IDNM_005392.3
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Entrez GenePHF2 (a.k.a. CENP-35, GRC5, JHDM1E, KDM7C)
- Promoter TRE promoter, Tet ON
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer LNCX (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLIX402 caPHF2 was a gift from Bob Weinberg (Addgene plasmid # 91701 ; http://n2t.net/addgene:91701 ; RRID:Addgene_91701) -
For your References section:
Activation of PKA leads to mesenchymal-to-epithelial transition and loss of tumor-initiating ability. Pattabiraman DR, Bierie B, Kober KI, Thiru P, Krall JA, Zill C, Reinhardt F, Tam WL, Weinberg RA. Science. 2016 Mar 4;351(6277):aad3680. doi: 10.1126/science.aad3680. 10.1126/science.aad3680 PubMed 26941323