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PurposeExpression of CjCas9 with His tag in E.coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 89754 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28b
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Backbone manufacturerGenescript
- Backbone size w/o insert (bp) 5400
- Total vector size (bp) 8365
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Vector typeBacterial Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Growth instructionsTransform into BL21(DE3) for protein expression and purification purposes
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCjCas9
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Alt nameCjeCas9
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SpeciesCampylobacter jejuni
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Insert Size (bp)3048
- Promoter T7
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Tags
/ Fusion Proteins
- 6XHis (N terminal on backbone)
- HA (N terminal on backbone)
- SV40 NLS (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site SacI (destroyed during cloning)
- 5′ sequencing primer taatacgactcactatagg (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-CjCas9 was a gift from Seokjoong Kim (Addgene plasmid # 89754 ; http://n2t.net/addgene:89754 ; RRID:Addgene_89754) -
For your References section:
In vivo genome editing with a small Cas9 orthologue derived from Campylobacter jejuni. Kim E, Koo T, Park SW, Kim D, Kim K, Cho HY, Song DW, Lee KJ, Jung MH, Kim S, Kim JH, Kim JH, Kim JS. Nat Commun. 2017 Feb 21;8:14500. doi: 10.1038/ncomms14500. 10.1038/ncomms14500 PubMed 28220790