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Addgene

pET28a(+)/FbGH30
(Plasmid #86463)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86463 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a(+)
  • Backbone manufacturer
    EMD Millipore
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 6812
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    FbGH30
  • Species
    Formosa B
  • Insert Size (bp)
    1443
  • GenBank ID
    AOR29490.1
  • Promoter T7
  • Tag / Fusion Protein
    • His-Tag (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CTCACAGCATGTACATCTAAAG
  • 3′ sequencing primer TTAGTTTGGTAATACAATCGTTTG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a(+)/FbGH30 was a gift from Jan-Hendrik Hehemann (Addgene plasmid # 86463 ; http://n2t.net/addgene:86463 ; RRID:Addgene_86463)

  • For your References section:

    Accurate quantification of laminarin in marine organic matter with enzymes from marine microbes. Becker S, Scheffel A, Polz MF, Hehemann JH. Appl Environ Microbiol. 2017 Feb 17. pii: AEM.03389-16. doi: 10.1128/AEM.03389-16. 10.1128/AEM.03389-16 PubMed 28213541
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