Skip to main content
Addgene
Open Menu

Human ICAM-1
(Plasmid #8632)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8632 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart
DNA 8632-D50 50 μg of DNA in Tris buffer $495
Add to Cart
DNA 8632-D100 100 μg of DNA in Tris buffer $585
Add to Cart

Backbone

  • Vector backbone
    pCDM8
  • Backbone manufacturer
    BCCM/LMBP
  • Backbone size w/o insert (bp) 4356
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Tetracycline, 100 & 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    MC1061/P3
  • Growth instructions
    The host strain is MC1061/P3 and the bacteria should be cultured in 12.5ug/mL ampicillin and 16ug/mL tetracycline. (supF)
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ICAM-1
  • Alt name
    CD54
  • Alt name
    pCD1.8
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1800
  • GenBank ID
    NM_000201.1
  • Entrez Gene
    ICAM1 (a.k.a. BB2, CD54, P3.58)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This clone will direct the expression of functional human ICAM-1 in a variety of cell types. This sequence has been deposited in Genbank with accession number J03132. Also see refs: Nature 329, 840-842 (1987) and Nature 339, 61-64 (1989)

Please use either GenBank IDs NM_000201.1 or J03132.1 as a refence sequence for the ICAM-1 insert in this plasmid. GenBank ID NM_000201.2 (updated after this plasmid was created) is not accurate for comparison.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human ICAM-1 was a gift from Timothy Springer (Addgene plasmid # 8632 ; http://n2t.net/addgene:8632 ; RRID:Addgene_8632)

  • For your References section:

    Primary structure of ICAM-1 demonstrates interaction between members of the immunoglobulin and integrin supergene families. Staunton DE, Marlin SD, Stratowa C, Dustin ML, Springer TA. Cell 1988 Mar 25;52(6):925-33. 10.1016/0092-8674(88)90434-5 PubMed 3349522
Related items:
Commonly requested with: