pH3F-PYK1
(Plasmid
#85774)
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PurposeN-ICE plasmid pH3F-PYK1
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 85774 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUG6
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Backbone manufacturerEuroscarf
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Vector typeYeast Expression, Cre/Lox
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameloxP-HphMX-loxP-PYK1p-3FLAG
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SpeciesS. cerevisiae (budding yeast), Synthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SpeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer Spt6 promoter (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pH3F-PYK1 was a gift from Scott Briggs (Addgene plasmid # 85774 ; http://n2t.net/addgene:85774 ; RRID:Addgene_85774) -
For your References section:
N-ICE plasmids for generating N-terminal 3 x FLAG tagged genes that allow Inducible, Constitutive, or Endogenous expression in Saccharomyces cerevisiae. Zhang Y, Serratore ND, Briggs SD. Yeast. 2016 Dec 12. doi: 10.1002/yea.3226. 10.1002/yea.3226 PubMed 27943405