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PurposeshRNA against mouse Tet3
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 85740 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAAV
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTet3
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gRNA/shRNA sequence5’-GCTCCAACGAGAAGCTATTTG-3’
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SpeciesM. musculus (mouse)
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Entrez GeneTet3 (a.k.a. B430006D22Rik, D230004J03Rik)
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Tag
/ Fusion Protein
- EYFP (C terminal on insert)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAV-shRNA_Tet3 was a gift from Hongjun Song (Addgene plasmid # 85740 ; http://n2t.net/addgene:85740 ; RRID:Addgene_85740) -
For your References section:
Tet3 regulates synaptic transmission and homeostatic plasticity via DNA oxidation and repair. Yu H, Su Y, Shin J, Zhong C, Guo JU, Weng YL, Gao F, Geschwind DH, Coppola G, Ming GL, Song H. Nat Neurosci. 2015 Jun;18(6):836-43. doi: 10.1038/nn.4008. Epub 2015 Apr 27. 10.1038/nn.4008 PubMed 25915473