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Addgene

pAAV-hSyn-FLEX-TVA-P2A-EGFP-2A-oG
(Plasmid #85225)

Ordering

This material is available to academics and nonprofits only. Orders shipped outside the U.S. may require additional regulatory approval, as well as a non-refundable export license fee of $85. Please log in to view availability.
Item Catalog # Description Quantity Price (USD)
Plasmid 85225 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pAAV-syn-FLEX-splitTVA-eGFP-B19G IW
  • Vector type
    AAV, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Optimized G Protein
  • Alt name
    oG
  • Species
    Synthetic
  • Insert Size (bp)
    1575
  • Promoter hSyn

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site BsrGI (not destroyed)
  • 5′ sequencing primer AGTCGTGTCGTGCCTGAGAG
  • 3′ sequencing primer CGGGATCACTCTCGGCATGG
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Enhanced GFP
  • Alt name
    EGFP
  • Species
    Synthetic
  • Insert Size (bp)
    717

Gene/Insert 3

  • Gene/Insert name
    TVA
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    468
  • Entrez Gene
    CD320 (a.k.a. TVA)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Optimized G protein was provide by Euiseok Kim and Ed Callaway at the Salk Institute.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-hSyn-FLEX-TVA-P2A-EGFP-2A-oG was a gift from John Naughton (Addgene plasmid # 85225 ; http://n2t.net/addgene:85225 ; RRID:Addgene_85225)