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Addgene

pFLAG-CMV2-Hsp27-S15D/S78D/S82D
(Plasmid #85188)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85188 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pFLAG-CMV-2
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Hsp27
  • Species
    H. sapiens (human)
  • Mutation
    S15D/S78D/S82D, phosphomimicking mutation
  • Entrez Gene
    HSPB1 (a.k.a. CMT2F, HEL-S-102, HMN2B, HMND3, HS.76067, HSP27, HSP28, Hsp25, SRP27)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The pFLAG-CMV2-Hsp27 wild-type plasmid was obtained from Dr. Park and is described by Park KJ, Gaynor RB, Kwak YT. J Biol Chem 2003; 278:35272-35278. PMID:12829720

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFLAG-CMV2-Hsp27-S15D/S78D/S82D was a gift from Ugo Moens (Addgene plasmid # 85188 ; http://n2t.net/addgene:85188 ; RRID:Addgene_85188)