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Addgene

hAur-A
(Plasmid #8510)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8510 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBABE-puro
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hAur-A
  • Alt name
    Aurora A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1200
  • Entrez Gene
    AURKA (a.k.a. AIK, ARK1, AURA, BTAK, PPP1R47, STK15, STK6, STK7)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer pBABE 5'
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map. For production of retrovirus expressing human Aur-A wild-type. (Ruderman RC232)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hAur-A was a gift from Joan Ruderman (Addgene plasmid # 8510 ; http://n2t.net/addgene:8510 ; RRID:Addgene_8510)
  • For your References section:

    Requirements for the destruction of human Aurora-A. Crane R, Kloepfer A, Ruderman JV. J Cell Sci 2004 Dec 1;117(Pt 25):5975-83. Epub 2004 Nov 9. 10.1242/jcs.01418 PubMed 15536123