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Purposeexpress beta-galactosidase in eukaryotic cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 83943 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEQ3
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Modifications to backbonepEQ3 is a promoterless beta-galactosidase (beta-gal) expression plasmid derived from pON1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCMV enhancer/promoter
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SpeciesCMV
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Insert Size (bp)1100
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GenBank IDX17403
- Promoter CMV
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Tag
/ Fusion Protein
- beta-galactosidase (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site PstI (unknown if destroyed)
- 3′ cloning site HindIII (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEQ176 was a gift from Adam Geballe (Addgene plasmid # 83943 ; http://n2t.net/addgene:83943 ; RRID:Addgene_83943) -
For your References section:
Translational control of human cytomegalovirus gp48 expression. Schleiss MR, Degnin CR, Geballe AP. J Virol. 1991 Dec;65(12):6782-9. PubMed 1658374