-
PurposeGateway Donor vector containing LIG1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.
-
Depositing Labs
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 83006 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepDONR223
-
Backbone manufacturerInvitrogen
-
Vector typeGateway Entry vector
Growth in Bacteria
-
Bacterial Resistance(s)Spectinomycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameLIG1
-
SpeciesH. sapiens (human)
-
GenBank IDNM_000234
-
Entrez GeneLIG1 (a.k.a. IMD96, LIGI, hLig1)
- Promoter None
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13pUC-fwd
- 3′ sequencing primer M13-Rev (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Open state: NO stop codon immediately follows insert
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pDONR223_LIG1_WT_V5 was a gift from Jesse Boehm & Matthew Meyerson & David Root (Addgene plasmid # 83006 ; http://n2t.net/addgene:83006 ; RRID:Addgene_83006) -
For your References section:
High-throughput Phenotyping of Lung Cancer Somatic Mutations. Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS. Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28. 10.1016/j.ccell.2016.06.022 PubMed 27478040