DNMT3ACD-CRY2-EGFP
(Plasmid
#82556)
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PurposeEncodes DMNT3 under LITE2.0 system
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 82556 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV_EF1a_WPRE_hGHpA
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Backbone manufacturerZhang lab
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameDNMT3A
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SpeciesH. sapiens (human)
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Entrez GeneDNMT3A (a.k.a. DNMT3A2, HESJAS, M.HsaIIIA, TBRS)
- Promoter EF-1alpha
Cloning Information for Gene/Insert 1
- Cloning method Restriction Enzyme
- 5′ cloning site Acc651 (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameCRY2
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SpeciesA. thaliana (mustard weed)
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MutationCRY2PHR (N-terminal photolyase homology region domain of CRY2 only)
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Entrez GeneCRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
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Tags
/ Fusion Proteins
- NLS(alpha-imp) (N terminal on backbone)
- NLS-VP64 (C terminal on backbone)
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer unknown (Common Sequencing Primers)
Gene/Insert 3
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Gene/Insert nameEGFP
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Speciesjellyfish
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Tag
/ Fusion Protein
- 2A (N terminal on backbone)
Cloning Information for Gene/Insert 3
- Cloning method Unknown
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe insert (DNMT3A) was incorporated into the ‘pAAV_EF1α_WPRE_hGHpA’ backbone of mammalian expression vector (plasmid #47457, from Zhang F, Addgene).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
DNMT3ACD-CRY2-EGFP was a gift from Joseph Irudayaraj (Addgene plasmid # 82556 ; http://n2t.net/addgene:82556 ; RRID:Addgene_82556) -
For your References section:
Epigenetic Editing of Ascl1 Gene in Neural Stem Cells by Optogenetics. Lo CL, Choudhury SR, Irudayaraj J, Zhou FC. Sci Rep. 2017 Feb 9;7:42047. doi: 10.1038/srep42047. 10.1038/srep42047 PubMed 28181538