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Addgene

DNMT3ACD-CRY2-EGFP
(Plasmid #82556)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 82556 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pAAV_EF1a_WPRE_hGHpA
  • Backbone manufacturer
    Zhang lab
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    DNMT3A
  • Species
    H. sapiens (human)
  • Entrez Gene
    DNMT3A (a.k.a. DNMT3A2, HESJAS, M.HsaIIIA, TBRS)
  • Promoter EF-1alpha

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site Acc651 (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    CRY2
  • Species
    A. thaliana (mustard weed)
  • Mutation
    CRY2PHR (N-terminal photolyase homology region domain of CRY2 only)
  • Entrez Gene
    CRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
  • Tags / Fusion Proteins
    • NLS(alpha-imp) (N terminal on backbone)
    • NLS-VP64 (C terminal on backbone)

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    EGFP
  • Species
    jellyfish
  • Tag / Fusion Protein
    • 2A (N terminal on backbone)

Cloning Information for Gene/Insert 3

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The insert (DNMT3A) was incorporated into the ‘pAAV_EF1α_WPRE_hGHpA’ backbone of mammalian expression vector (plasmid #47457, from Zhang F, Addgene).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DNMT3ACD-CRY2-EGFP was a gift from Joseph Irudayaraj (Addgene plasmid # 82556 ; http://n2t.net/addgene:82556 ; RRID:Addgene_82556)
  • For your References section:

    Epigenetic Editing of Ascl1 Gene in Neural Stem Cells by Optogenetics. Lo CL, Choudhury SR, Irudayaraj J, Zhou FC. Sci Rep. 2017 Feb 9;7:42047. doi: 10.1038/srep42047. 10.1038/srep42047 PubMed 28181538