pGEX-6P-1-GST-ATL(Cbx7)
(Plasmid
#82526)
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PurposeExpresses GST-CD (Cbx7) fusion proteins in E. coli. Cbx7 ATL region only, amino acids 66–81.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 82526 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEX-6P-1
- Backbone size w/o insert (bp) 4950
- Total vector size (bp) 5027
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameChromobox Homolog 7
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Alt nameCbx7
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SpeciesH. sapiens (human)
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Insert Size (bp)51
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MutationATL(Cbx7), amino acids 66–81
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GenBank IDNM_175709
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Entrez GeneCBX7
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Tag
/ Fusion Protein
- GST (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (unknown if destroyed)
- 3′ cloning site Xho1 (unknown if destroyed)
- 5′ sequencing primer CACGTTTGGTGGTGGCGACCATCCTCCAA
- 3′ sequencing primer ATGACGGTGAAAACCTCTGACACAT (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX-6P-1-GST-ATL(Cbx7) was a gift from Xiaojun Ren (Addgene plasmid # 82526 ; http://n2t.net/addgene:82526 ; RRID:Addgene_82526)