pOTTC841 - pAAV minP secNLuc
(Plasmid
#82498)
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PurposeLongitudinal monitoring of Gaussia and Nano luciferase activities to simultaneously assess ER calcium homeostasis and ER stress in vivo
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 82498 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV EF1a
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Backbone manufacturerKarl Deisseroth
- Backbone size w/o insert (bp) 5637
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesecNLuc
- Promoter c-Fos minimal promoter
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer NA
- 3′ sequencing primer WPRE_R1 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOTTC841 - pAAV minP secNLuc was a gift from Brandon Harvey (Addgene plasmid # 82498 ; http://n2t.net/addgene:82498 ; RRID:Addgene_82498) -
For your References section:
Longitudinal monitoring of Gaussia and Nano luciferase activities to concurrently assess ER calcium homeostasis and ER stress in vivo. Wires ES, Henderson MJ, Yan X, Back S, Trychta KA, Lutrey MH, Harvey BK. PLoS One. 2017 Apr 12;12(4):e0175481. doi: 10.1371/journal.pone.0175481. eCollection 2017. 10.1371/journal.pone.0175481 PubMed 28403212