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Addgene

rFIXX-bp chain B (IRESpuro)
(Plasmid #82451)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 82451 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pIRES
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5115
  • Total vector size (bp) 5638
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rFIXX-bp chain B
  • Alt name
    Recombinate Factor IX/X binding protein chain B
  • Species
    Trimeresurus flavoviridis
  • Insert Size (bp)
    523
  • Promoter T7 RNA polymerase promoter
  • Tag / Fusion Protein
    • Biotin (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer Unknown
  • 3′ sequencing primer Unknown
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    rFIXX-bp chain B (IRESpuro) was a gift from Robert Peters (Addgene plasmid # 82451 ; http://n2t.net/addgene:82451 ; RRID:Addgene_82451)